Abstract

High risk types of Human Papillomavirus are the causative agents of virtually all cases of cervical cancer, 50-90% of other anogenital cancers and approximately 30% of oral and pharyngeal cancers. The high-risk types encode two viral oncogenes, E6 and E7, which work together to initiate cell transformation. The approximately 50 amino acid product of the E6* transcript is expressed during the early stages of HPV infection. In this study, we found that expression of E6* increased the level of reactive oxygen species (ROS) in both HPV+ and HPV- cells. This increased oxidative stress led to higher levels of DNA damage. The observed increase in ROS may be due to a decrease in cellular anti-oxidant activity, as we found that E6* expression also led to decreased expression of SOD and Gpx, These studies indicate that E6* may play an important role in virus-induced mutagenesis by increasing oxidative stress and DNA damage.

LLU Discipline

Biochemistry

Department

Basic Sciences

School

School of Medicine

First Advisor

Duerksen-Hughes, Penelope J.

Second Advisor

Brantly, Eileen

Third Advisor

Payne, Kimberly J.

Fourth Advisor

Reeves, Mark E.

Fifth Advisor

Soto-Wegner, Ubaldo

Degree Name

Doctor of Philosophy (PhD)

Degree Level

Ph.D.

Year Degree Awarded

2014

Date (Title Page)

6-2014

Language

English

Library of Congress/MESH Subject Headings

Human Papillomavirus 16; Papillomavirus Infections; Uterine Cervical Neoplasms; Mouth Neoplasms

Subject - Local

Human Papillomavirus 16; Cervical Cancer; Anogenital Cancer; Oral and Pharyngeal Cancers; Virus-Induced Mutagenesis; Oxidative Stress; DNA Damage

Type

Dissertation

Page Count

109

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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