Systemic Lupus Erythematosus and Rheumatoid Arthritis are B cell-mediated autoimmune diseases that afflict millions of people worldwide. B cell-targeted therapies for these diseases result in variable clinical outcomes. Thus, a need exists to better understand the dynamics of human B cell production and function. The mouse model has provided a foundation for understanding the mechanisms involved in human B cell development and autoimmune disease. However, differences in mouse and human B cells are not fully understood. Our work shows that the co-expression of CD21 and CD24, determined by 7-color flow cytometry, can be used to demarcate developmental subsets of B cells. A comparison of analogous B cell subsets in mice and humans showed that the B cell subsets distribution differs between the species suggesting differences exist in mechanisms that regulate and maintain the specific B cell. This work provides a foundation for understanding human B cell development using the mouse model.
Microbiology and Molecular Genetics
School of Medicine
Payne, Kimberly J.
Casiano, Carlos A.
Colburn, Keith K.
Doctor of Philosophy (PhD)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Antigens - Differentiation; Antigens - CD24; Receptors - Complement 3d; B-Lymphocyte Subset; B-Cell Activating Factor; Lymphoid Progenitor Cells; Hematopoietic Stem Cells; Immunoglobulin Light Chains; Common Variable Immunodeficiency; Species Specificity
Subject - Local
Systemic Lupus Erythematosus; Rheumatoid Arthritis; B cell-mediated autoimmune diseases; Mouse model; Human B Cell development;
Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Benitez, Abigail, "CD21 and CD24 Co-expression: A Translational Model between Mouse and Human" (2014). Loma Linda University Electronic Theses, Dissertations & Projects. Paper 198.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives