Abstract

An ideal characteristic of root-end filling materials used in endodontics is the ability to enhance hard tissue formation. Studies have shown that MTA has the potential to enhance hard tissue formation when placed in contact with periradicular tissues. The mechanisms involved in hard tissue formation of MTA and other root-end filling materials have not been completely elucidated. The purpose of this investigation was to study the mechanism of hard tissue formation of commonly used root-end filling materials and MTA by evaluating alkaline phosphatase (ALP) activity and cell layer protein. Extracts of materials tested in this experiment were added to the medium of human osteoblast-like osteosarcoma cell strain, SaOS-2 and cultured for 48 hours. Water incubated in parallel served as the negative control. 1,25-Dihydroxyvitaim D3 was added to the cells as the positive control. ALP activity and protein concentration were assayed in two separate experiments to study effect of materials on the cells in Experiment 1, as well as a dose response relationship in Experiment 2. Data was collected and analyzed. Based on our results it appears that hard tissue formation by MTA is not directly related to ALP activity.

Department

Dentistry

School

Graduate School

First Advisor

Mahmound Torabinejad

Second Advisor

John Farley

Third Advisor

Thomas Linkhart

Fourth Advisor

Shahrokh Shabahang

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2001

Date (Title Page)

6-2001

Language

English

Library of Congress/MESH Subject Headings

Dental Pulp Capping; Dental Materials; Root Canal Filling Materials

Type

Thesis

Page Count

ix; 56

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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