Abstract

Enterococcus faecalis is the most commonly isolated bacteria in failed root canal treatment. Endodontic intracanal medicaments are commonly tested using standardized bovine cylinders infected with bacteria. The literature is not clear on whether calcium hydroxides are able to remove E. faecalis from the dentinal tubules. One reason for this is that there are several varying forms of calcium hydroxide commonly used. The purpose of the present experiment was to determine whether four commonly used calcium hydroxide products were able to reduce E. faecalis from four incremental samples of dentin and to determine if there was a statistically significant difference between each medicament in its ability to eliminate E. faecalis from bovine dentinal tubules.

Ninety freshly extracted incisors from 22-24 month old cows were milled into standardized cylinders of the following dimension: 5 mm high, external diameter of 7 mm, and lumen diameter of 2.7 mm. The cylinders were sterilized and were all subsequently infected with Enterococcus faecalis ATCC4082, except for 15 which were set aside as negative controls. The remaining cylinders were divided into four treatment groups of 15 and a positive control group also consisting of 15 cylinders. The four treatment groups had the canal lumen filled with Calasept®, Diapex®, Ultracal®, or Pulpdent® for two weeks. After two weeks, round burs sized ISO 029, 031, 033, and 035 were passed through the lumens, dentin shavings were weighed, cultured and CFU/mg were enumerated.

One-way ANOVA, Student-Newman-Keuls multiple comparison procedure, and Kruskal-Wallace tests were used for data analysis. Statistically significant reductions in CFU/mg E. faecalis were found between the four treatment groups and the positive controls (p < 0.0001). Reductions in CFU/mg E. faecalis of 94.1%-100.0% were observed. There was no statistically significant difference between the medicaments. All p-values were > 0.5.

LLU Discipline

Pediatric Dentistry

Department

Pediatric Dentistry

School

Graduate Studies

First Advisor

John Peterson

Second Advisor

Leif K. Bakland

Third Advisor

James D. Kettering

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2006

Date (Title Page)

9-2006

Language

English

Library of Congress/MESH Subject Headings

Dentin; Dentin-Bonding Agents; Calcium Hydroxide; Root Canal Therapy; Enterococcus Faecalis

Type

Thesis

Page Count

ix; 30

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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