Abstract

Diabetes Mellitus (DM) has been implicated as a factor affecting healing. The purpose of this study was to use the healing of experimentally exposed pulps subsequent to pulp capping as a model to determine the effect of DM on healing.

Twenty-two Sprague-Dawley rats were divided into two groups of eleven animals each. In one group, DM was induced by injection of 70 mg/KG of streptozotocin. In the other group, the animals were injected with sterile saline. Under anesthesia with Ketamine and Xylazine, the pulps of the maxillary first molars of all rats were exposed using a 1/16 round bur under continuous water spray. After achieving hemostasis with sterile paper points and 5.25% sodium hypochlorite, the exposure sites were sealed with mineral trioxide aggregate (MTA). Intact teeth and teeth with exposed pulps without restorations in both groups served as positive and negative controls, respectively. All animals were sacrificed four weeks after pulp capping. Histological samples were prepared and evaluated for dentin bridge formation and pulpal inflammation. Data was analyzed using Fisher's Exact, Mann-Whitney U, and Spearman's correlation tests. Complete dentin bridge formation was significantly inhibited in diabetic rats (p = 0.029). The pulps in the diabetic rats were significantly more inflamed following the pulp capping procedure, compared to non-diabetic animals (p = 0.005). There was a significant inverse association between dentin bridge formation and inflammatory cell infiltration (p = 0.001). Based on the results of this study, it appears that DM has a major impact on pulpal healing in rats.

Department

Dentistry

School

Graduate Studies

First Advisor

Mahmoud Torabinejad

Second Advisor

George Lessard

Third Advisor

Shahrokh Shabahang

Fourth Advisor

Kenneth Wright

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2006

Date (Title Page)

6-2006

Language

English

Library of Congress/MESH Subject Headings

Diabetes Mellitus; Dental Care; Wound Healing; Dental Pulp; Endodontics

Type

Thesis

Page Count

ix; 29

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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