This thesis describes an in vivo perfusion apparatus which can continuously deliver controlled quantities of solutions to localized, contralateral intra oral areas in albino rats. Three animals were per fused on the experimental area (the mesial sulcular area of the maxillary first molar) on one side with tap water and trypan blue. Localization of the solution delivered and minimal histological changes due to the presence of the apparatus was demonstrated on these animals. A 1% solution of trypsin was perfused on the experimental area of one animal for one day at the rate of 4.8 ml/day and on another animal for four days at the same rate. A 1% pronase solution was perfused for four days on one animal at the rate of .96 ml/day and another animal at 4.8 ml/day. The appropriate control solutions were delivered on the contralateral side of each animal undergoing enzyme perfusion. Data obtained indicated moderate inflammatory changes were noted on the side receiving the enzyme. More meaningful results should be obtained with the modifications discussed in this report.
Richard C. Oliver
Edwin M. Collins
G. Conrad Hornbuckle
John De Vincenzo
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
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Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Dornan, Don, "An In Vivo Perfusion Apparatus for Studies on Gingival Disease : Assessment by a Perfusion of Nonspecific Proteolytic Enzymes" (1969). Loma Linda University Electronic Theses, Dissertations & Projects. 1189.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives