Trichophyton rubrum was grown on a chemically defined media in an effort to find a substance that would give the maximum production of macroconidia. Twenty-one amino acids were tested and about one half of them were active in stimulating macroconidial formation. Eight purines and pyrimidines were also tested but none of them gave more than scanty sporulation. The base media used was Czapek solution agar (modified): the saccharose was replaced with an equal amount of glucose. The various nutrients were tested at two concentrations, 1.25 mg/ml and 0.25 mg/ml. Yeast extract and peptone were also included in the study since these complex mixtures are both known to increase growth and promote sporulation of some of the dermatophytes.
The color of the colonies varied greatly and ranged from colorless on the base media to a deep red. Some of the mounts had large reddish-orange crystals, believed to be crystalized pigment.
Five of the amino acids produced abundant sporulation, and had significantly higher counts of macroconidia than either yeast extract or peptone. Aspartic acid was outstandingly effecient; the number of macroconidia per square millimeter was more than four times greater than any of the other nutrients.
Additional investigations are proposed to compare the reaction of other strains and to find the best combination for good growth and maximum sporulation.
Ian M. Fraser
Yuk Lin Ho
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This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Austin, P. Jerry, "The Effect of Various Nutrients on the Sporulation of TRICHOPHYTON RUBRUM" (1972). Loma Linda University Electronic Theses, Dissertations & Projects. 1408.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives