Adults of the blood fluke Schistosoma mansoni were perfused from the hepatic portal system of CF1 female mice sixty to seventy days post infection. The worms were then incubated in Eagle’s minimal essential medium with glutamate and 0.03 mg/100 ml of nonlabelled aspartic acid. To this medium was added 10 μc of either NaH1403, glucose-UC14 or aspartate-4-C14. The worms were found to fix carbon from the NaH1403 and to incorporate the glucose and aspartate. The amount of activity recovered in five different chemical fractions after two hours incubation was determined for each radiochemical used and compared to determine the relative significance of carbon dioxide in Schistosoma mansoni metabolism.
All five fractions which were separated from worms incubated in glucose were significantly different from the control samples. The worms incubated in aspartate and sodium bicarbonate incorporated these compounds into every fraction except the ethanol fraction. Worms incubated in both aspartate and sodium bicarbonate had the greatest amount of incorporation of label in the protein fraction with the least amount in the ethanol soluble fraction.
Under the experimental conditions employed glucose appears to be the most important source of carbon when compared with either aspartate or bicarbonate.
Ariel A. Roth
Edward D. Wagner
R. Bruce Wilcox
Master of Arts (MA)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Carbon Dioxide; Schistosoma mansoni
Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Carter, Clint Earl, "Carbon Dioxide Fixation in Schistosoma Mansoni" (1967). Loma Linda University Electronic Theses, Dissertations & Projects. 1451.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives