The effect of 9α-FF on the Na:K ratio, chloride, and pH of saliva and gastric secretions, and on the total and free acid of gastric juice in normal human subjects was investigated. This study was designed to investigate whether conditions could be produced in the normal human subject similar to those present in patients with primary aldosteronism by using large doses of 9α-FF. August and others found a change in salivary electrolytes when cortisone, desoxycortisone, or ACTH were given to normal subjects. In these cases as in primary aldosteronism there was a decreased Na:K ratio and potassium excretion, with sodium retention. There were no reports in the reviewed literature which stated effects of steroid on gastric secretions of human subjects.
Saliva and gastric juice specimens stimulated by Histalog® were obtained prior to and after administration of 2 milligrams per day of 9α-FF acetate for 2 days. Sodium and potassium were measured photometrically; chloride was calculated by the Schales' method; total and free acid were estimated by the method of Topfer, and pH was read on a Beckman pH meter.
Salivary sodium, potassium, chloride and pH did not change significantly during administration of 9α-FF. The majority of samples showed an increase in potassium concentration and a decrease in sodium concentration when samples collected at the end of steroid administration were compared with pre-steroid samples for the same subject.
Sodium, chloride, pH, total and free acid of gastric juice did not vary significantly during 9α-FF medication. Potassium concentration increased 9.7 ±1.5 to 11.4 ± 2.3 milliequivalents per liter in samples not influenced by Histalog.®
It was concluded from this study of normal human subjects that 2 milligrams per day of 9α-FF produced the same effect on salivary electrolytes as cortisone, desoxycorticosterone or aldosterone; however, not the same degree of change was presented that has been recorded by other investigators. Statistical significance of change was not established due to the large standard deviations. The increased potassium concentration of gastric secretions during 9α-FF was significant. It is believed that uncontrollable variables prohibited the sample from reaching statistically significant changes. Further studies of the same type should be carried out over extended periods of time to confirm changes in saliva and gastric juice observed in this study.
Milton G. Crane
Arthur J. Riesenfeld
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Saliva; Gastric Juice; Mineralocorticoids -- analysis.
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This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Beckner, Ardis Stern, "Effect of 9 α-fluorohydrocortisone Acetate on Gastric and Salivary Electrolytes of Normal Fasting Subjects" (1962). Loma Linda University Electronic Theses, Dissertations & Projects. 1505.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives