This thesis describes an in vivo perfusion apparatus which can continuously deliver controlled quantities of solutions to localized, contralateral intraoral areas in albino rats. Three animals were per fused on the experimental area (the mesial sulcular area of the maxillary first molar) on one side with tap water and Trypan blue. Localization of the solution delivered and minimal histological changes due to the presence of the apparatus was demonstrated on these animals. Solutions of .5%, 1%, and 2% hyaluronidase and collagenase were perfused on the experimental areas of nine animals. When both enzymes were used, hyaluronidase always preceded collagenase in sequence. Rate of flow ranged from 4.8 to 7.1 ml/day. The appropriate control solutions were delivered on the contralateral side of each animal undergoing enzyme perfusion. Data obtained from animals perfused with collagenase for periods over sixty hours at from 1% to 2% concentration indicated moderate to severe inflammatory changes on the experimental side. More meaningful results should be obtained with the modifications discussed in this report
Richard C. Oliver
Edwin M. Collins
G. Conrad Hornbuckle
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Gingival Diseases; Dental Equipment
Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Smith, James A., "An In Vivo Perfusion Apparatus for Studies on Gingival Disease in the Albino Rat : Assessment by a Perfusion of the Enzymes Collagenase and Hyaluronidase" (1970). Loma Linda University Electronic Theses, Dissertations & Projects. 1625.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives