It has been shown previously that a root surface which has been scaled, root planed and demineralized by topical application of citric acid in vitro, is dominated by exposed collagen fibrils. The purpose of this study was to examine the mechanism of healing and possible reattachment of soft periodontal connective tissue to the root planed and subsequently acid treated root surfaces and to determine whether new collagen fibrils produced in the connective tissue could attach to these exposed fibrils.
Full thickness tissue flaps were raised and the coronal 4-5 mm of the labial alveolar plate removed over the maxillary incisor roots of 7 Labrador retriever dogs providing 28 teeth for study. The exposed root surfaces were then planed to remove all cementum and some dentin before etching with citric acid (pH 1) for 3 min. The operation area was thoroughly irrigated and the flap reapposed and sutured in place at its original level. Healing was followed for 7, 14, 21 and 42 days at which times block sections were harvested and processed for transmission electron microscopic study. The findings appear to be consistent with previous reports of improved tissue regeneration with the use of citric acid.
The mechanism of healing of connective tissue to the root surface appears to be by way of interdigitation of newly produced collagen fibrils with the preexisting collagen fibrils of the exposed dentin matrix.
Knut A. Selvig
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Tooth Root; Wound Healing
Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Ririe, Craig Martin, "Ultrastructural Study of Wound Healing on a Root Planed and Citric Acid Treated Root Surface" (1978). Loma Linda University Electronic Theses, Dissertations & Projects. 2061.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives