The objective of this study was to evaluate bone and cementum regeneration in the critical size, supraalveolar, periodontal defect model following surgical implantation of recombinant human transforming growth factor beta-1 (rhTGFß-1) in conjunction with guided tissue regeneration (GTR). Five male beagle dogs were used in a split-mouth design. Alternate jaw quadrants in consecutive animals received GTR with rhTGFß-1 in a resorbable calcium carbonate carrier. Contralateral jaw quadrants received GTR with carrier only (control). The healing interval was 4 weeks. Clinical healing was generally uneventful, however, minor membrane exposures were observed for both treatments with limited gingival inflammation. All defects with exposed membranes exhibited an inflammatory infiltrate underneath the membrane. For all defects evaluated (including defects exhibiting an inflammatory infiltrate), bone regeneration (height and area) was numerically increased in rhTGFß-1 defects compared to control, however, the difference between treatments did not reach statistical significance. A statistically significant difference in bone density was found between rhTGFß-1 and control defects. When defects without signs of inflammation were evaluated separately, a statistically significant difference between treatments was found in area and density of regenerated bone. Comparisons of cementum regeneration, root resorption and ankylosis did not reveal any differences between treatments. Although statistically significant differences were observed between rhTGFß-1 and control treatments with respect to bone regeneration area and density, observed differences were of low magnitude and present an insignificant potential for enhancement of bone regeneration by rhTGFß-1 in this preclinical model. Within limitations of this study, it may be concluded that rhTGFß-1 has a limited potential to enhance alveolar bone and cementum regeneration in conjunction with GTR procedures.

LLU Discipline





Graduate School

First Advisor

Ulf M. E. Wikesjö

Second Advisor

Thorarinn J. Sigurdsson

Third Advisor

Philip J. Boyne

Degree Name

Master of Science (MS)

Degree Level


Year Degree Awarded


Date (Title Page)




Library of Congress/MESH Subject Headings

Periodontal Diseases -- surgery; Transforming Growth Factor beta; Guided Tissue Regeneration; Growth Substances



Page Count

2 vi; 49

Digital Format


Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.


Loma Linda University Electronic Theses and Dissertations

Collection Website



Loma Linda University. Del E. Webb Memorial Library. University Archives