We examined the major possible mechanisms for the left shift of the BK channel I-V relationship in native basilar artery myocytes from the two LTH groups. These mechanisms included: differential expression of the accessary BK -1 subunit; differential phosphorylation of the BK subunit; and splice variation of the BK subunit. Using molecular cloning, heterologous expression, and patch-clamp electrophysiology techniques, we elucidated a mechanism that, at least in part, contributes to the differences we observed between channels from native normoxic and LTH myocytes.
School of Medicine
Hessinger, David A.
Buchholz, John N.
Longo, Lawrence D.
Watts, Kylie J.
Doctor of Philosophy (PhD)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Anoxia; Hypoxia-Ischemia - Brain; Acclimatization; Altitude Sickness; Adaptation - Physiological; Myocytes; Potassium Channels - Calcium-Activated; Large-Conductance Calcium-activated Potassium Channels; Calcium Channel Blockers; Ion Channel Gating;
Subject - Local
Left Shift of the BK Channel; Basilar Artery Myocytes; Molecular Cloning; Heterologous Express; Patch-Clamp Electrophysiology;
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Tao, Xiaoxiao, "Acclimatization to High-Altitude, Long-Term Hypoxia Alters BK Channel Structure and Function" (2015). Loma Linda University Electronic Theses, Dissertations & Projects. 269.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives