Defining the role of cytokines in promoting human B cell development is important for understanding B cell leukemia and for developing strategies to restore B cell production that is lost during some therapies. The purpose of this study was to investigate the roles of the IL-7R ligands, thymic stromal lymphopoietin (TSLP) and IL- 7, in the early stages of normal postnatal and adult human B cell development. Using novel in vitro and in vivo model systems, we show that TSLP and IL-7 are able to expand B cell precursors individually and in combination from CB hematopoietic stem cells. In this model TSLP increases were seen in the CD34+ pro-B subset and maintained through subsequent stages of development. Adult B lymphopoiesis unlike CB B lymphopoiesis is IL-7– but may not be TSLP–dependent. We also used the expression of IL-7R, to identify a lymphoid progenitor that lacks myeloid potential. This information will be important in developing strategies for restoring B cell development after therapy and supports our ongoing leukemia studies by providing a rationale for targeting the TSLP signaling pathway in developing therapies for high-risk B cell acute lymphoblastic leukemia.
Microbiology and Molecular Genetics
School of Medicine
Payne, Kimberly J.
Casiano, Carlos A.
Duerksen-Hughes, Penelope J.
Doctor of Philosophy (Medical Science)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Cytokines -- Genetics; Cytokines -- Physiology; B Lymphocytes; Precursor Cell Lymphoblastic Leukemia-Lymphoma;
Subject - Local
Thymic Stromal Lymphopoietin (TSLP); IL-7R ligands; B Cell Development; Lymphoid Progenitor; Myeloid Potential
Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Milford, Terry-Ann, "Comparative Studies of TSLP and IL-7 in Normal Early Human Neonatal and Adult B Cell Development" (2016). Loma Linda University Electronic Theses, Dissertations & Projects. 402.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives