Aim: The aim of this in vitro disk diffusion susceptibility test is to compare the antibacterial activity of ozonated olive oil (OzOO) with chlorhexidine gluconate (CHX) against periodontal pathogens, Porphyromonas gingivalis and Aggregatibacter Actinomycetemcomitans. Materials and Methods: The antibacterial activity of two antiseptics, OzOO and CHX, and, two controls, olive oil and normal saline was assessed by disk diffusion susceptibility test. For disk diffusion susceptibility test, two periodontal pathogens, 20 petri dishes for P. gingivalis and 18 petri dishes for A. actinomycetemcomitans, were used. To assess the antibacterial activity, the diameters of the growth inhibition zone were measured with a caliper. Results: The diameters of the growth inhibition zone against P. gingivalis were 31.4 ± 5.05 mm for OzOO and 20.9 ± 1.19 mm for CHX (p<0.001). The diameters of the growth inhibition zone against A. actinomycetemcomitans were 25.9 ± 1.69 mm for OzOO and 18.4 ± 1.55 mm for CHX (p<0.001). All the measured diameters for olive oil and normal saline against both periodontal pathogens were 6 mm, with no sign of the growth inhibition. Conclusion: The antibacterial activity of OzOO against P. gingivalis and A. actinomycetemcomitans is superior to that of CHX.
School of Dentistry
Kim, Yoon Jeong
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Plant Oils -- Therapeutic use; dietary Fats; Unsaturated -- therapeutic use; Olive Oil -- Health aspects
Subject - Local
Antibacterial Activity; Chlorhexidine Gluconate Periodontal Pathogens
Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Lee, Changmin, "Comparison of Antibacterial Activity of Ozonated Olive Oil and Chlorhexidine Gluconate" (2017). Loma Linda University Electronic Theses, Dissertations & Projects. 515.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives