To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both promoters were active in all the conditions tested except during Beta-gal activity was higher in aerobic than in anaerobic growth; at 30°C and 37°C than at 42°C; and in L-broth early log phase of the culture. than in minimal media. Potential future uses of these fusion mutants are discussed.
Anthony J. Zuccarelli
Eric A. Goulbourne, Jr.
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
DNA Restriction Enzymes -- biosynthesis; Escherichia coli -- analysis.
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This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Prakash, Ainu, "The Expression of DNA Restriction-Modification Genes of ESCHERICHIA COLI K12" (1986). Loma Linda University Electronic Theses, Dissertations & Projects. 686.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives