The Effects of Biological Response Modification and Diet on the Immune Respose of Tumor-bearing mice

Author

David Grant Lovell

Abstract

The effect that diet and other modifiers may have upon the immune system of BALB/c mice bearing tumor were studied, mice were injected s.c. with 10⁶ herpes simplex virus Type 2- Adult male BALB/c transformed (H238) cells in the thigh causing a visible fibrosarcoma within 1-2 weeks. Mice were fed either a diet low in protein (milk) and low in fat (Diet 1), or another diet low in protein (wheat) and high in fat (Diet 8). Spleen cells were collected for assays of cytotoxic T-lynphocyte (CTL) and natural killer (NK) cell activity. At 3 weeks post-injection, a decrease in CTL activity against H238 tumor cells was observed but no change in NK activity against H238 or K562 targets was seen. Spleen cells were treated In vitro with mouse interferon, rat interleukin-2, 2-amino-5-bromo-6-methyl-4-pyrimidinone (ABMP), Prostaglandin E₁ (PGE₁), singly and in combination, for 2 hours prior to mixing with target cells. Treated spleen cells from Diet 1 tumor-bearing animals at 3-6 weeks post-injection had substantially increased CIL response while those from Diet 8 mice were only slightly enhanced. Interferon, interleukin-2 and prostaglandin E₁ most effective. NK activity remained essentially unchanged regardless were of diet group or modifying treatment.

LLU Discipline

Microbiology

Department

Microbiology

School

Graduate School

First Advisor

James D. Kettering

Second Advisor

Robert L. Nutter

Third Advisor

Daila S. Gridley

Fourth Advisor

George M. Lessard

Degree Name

Master of Arts (MA)

Degree Level

M.A.

Year Degree Awarded

1984

Date (Title Page)

6-1984

Language

English

Library of Congress/MESH Subject Headings

Immune System; Neoplasms -- diet therapy; Mice, Inbred BALB C

Type

Thesis

Page Count

viii; 84

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Copyright

Author

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Recommended Citation

Lovell, David Grant, "The Effects of Biological Response Modification and Diet on the Immune Respose of Tumor-bearing mice" (1984). Loma Linda University Electronic Theses, Dissertations & Projects. 725.
https://scholarsrepository.llu.edu/etd/725

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives