Mother’s milk is recommended by the nutritional and pediatric communities as the best food for suckling infants. For non breast-fed infants, bovine milk-based formulas have been regarded as a suitable substitute. In the early 1900's, physicians found that foreign proteins in cow milk were responsible for gastrointestinal disease and even digestive collapse of the newborn fed a cow milk-based diet16. The allergenicity of cow milk for the human infant appears to be caused predominantly by p-casein17. In this research, the cDNA encoding human p-casein was introduced into potato cells under the control of the bidirectional, auxin-inducible mannopine synthase gene (mas) promoter by Agrobacterium turnefaciens-mediated leaf disc transformation methods. The presence of the p-casein gene and its transcript in regenerated transgenic plants were confirmed by PCR and RT-PCR analysis respectively. Human p-casein protein was detected in leaves of transgenic plants by immunoblot analysis. The p-casein protein produced by the plants migrated as a single band with a molecular mass of about 30 KDa and was approximately 0.01% of the total soluble protein in transgenic potato tissue.
Microbiology and Molecular Genetics
Microbiology, Molecular Biology and Biochemistry
William H. R. Langrides
Allan P. Escher
Anthony J. Zuccarelli
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Milk Proteins; Milk, Human; Caseins; Plants, Transgenic; Plant Proteins
Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Roberts, Wendy, "Expression of the Human Milk Protein, Beta-Casein : in Transgenic Potato Plants" (1996). Loma Linda University Electronic Theses, Dissertations & Projects. 762.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives