An improved procedure for the quantitative estimation of urinary pregnanediol
Journal of Clinical Endocrinology and Metabolism
The estimation of urinary pregnanediol has been recommended as a test for pregnancy, and as an aid in predicting the fate of threatened abortion. In addition, it has been suggested that threatened abortion associated with diminished pregnanediol excretion may be averted by adequate progesterone therapy. The widespread interest in this determination has resulted in the description of various procedures adapted to clinical use. Among them are the methods of Guterman (1), Sommerville, Gough, and Marrian (2), Rabinovitch (3), and Huber (4). These are derived to a greater or lesser extent from the procedure of Guterman, who has combined the methods of Astwood and Jones (5) and of Talbot et al. (6) in a test suited to routine use. In our use of Guterman’s method over a period of time, we have introduced certain modifications which seem sufficiently important to warrant their being reported. In addition, we have determined the percentage recovery under the conditions of our procedure, so that a quantitative determination of pregnanediol excretion can readily be made. Some observations dealing with the rate of excretion of pregnanediol were obtained during the progress of this work, and are included in this report. © 1950 by The Endocrine Society.
Hoyt, Robert E. and Levine, Milton G., "An improved procedure for the quantitative estimation of urinary pregnanediol" (1950). LLU Faculty Publications. 185.