Abstract

A Klebsiella phage was isolated from the San Bernardino sewage and its physiochemical properties analyzed. The present study found the density of the phage to be 1.44 g/ml, the burst size to be 10, and the phage had a latent period of 20-25 minutes. The DNA was found to be double stranded and linear. Its molecular weight was 41.66 + 0.72 kilobase pairs. The DNA was digested with a battery of endoncleases and partial restriction maps determined. The potential of the phage as a vector for Klebsiella is discussed in the text.

LLU Discipline

Microbiology

Department

Microbiology

School

Graduate School

First Advisor

Leonard Bullas

Second Advisor

Richard Beltz

Third Advisor

James Kettering

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1984

Date (Title Page)

8-1984

Language

English

Library of Congress/MESH Subject Headings

Klebsiella pneumoniae -- analysis; DNA, Recombinant; Cloning, Molecular

Type

Thesis

Page Count

vi; 52

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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Included in

Microbiology Commons

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