Abstract
The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.
A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin (HA) response to a common Gram negative antigen (CA-) of Escherichia coli (014) was demonstrated in the rabbit when pre-incubated mixtures of CA(-) and M. arthritidis membranes were injected iv. A similar immunosuppression was demonstrated only for the secondary HA response to a common Gram positive antigen (CA+) of Staphylococcus aureus when pre-incubated mixtures of CA(+) and M. arthritidis membranes were employed as immunizing materials. Mixtures containing CA(+) appeared to interfere with MI antibody production in the rabbit.
The findings suggest 1) that oleic acid may play a role in the virulence of animal-passaged M. arthritidis in the rat# 2) that MI antibody synthesis may be subject to competitive inhibition by (CA+) in the rabbit, and 3) that the immunosuppressive effect of M. arthritidis is associated with membrane components and is not limited to the host in which arthritogenic properties are manifested.
LLU Discipline
Microbiology
Department
Microbiology
School
Graduate School
First Advisor
Ben H. S. Lau
Second Advisor
Charles E. Winter
Third Advisor
Robert L. Nutter
Fourth Advisor
Dick H. Koobs
Fifth Advisor
Walter E. Roop
Degree Name
Doctor of Philosophy (PhD)
Degree Level
Ph.D.
Year Degree Awarded
1973
Date (Title Page)
6-1973
Language
English
Library of Congress/MESH Subject Headings
Mycoplasmatales; Arthritis -- etiology; Antibody Formation
Type
Dissertation
Page Count
ill; 128
Digital Format
Digital Publisher
Loma Linda University Libraries
Copyright
Author
Usage Rights
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Recommended Citation
Bergquist, Lois M., "Role of Membrane-Associated Components of MYCOPLASMA ARTHRITIDIS on the Biosynthesis of Humoral Antibodies and Arthritogenic Properties in Experimental Animals" (1973). Loma Linda University Electronic Theses, Dissertations & Projects. 1305.
https://scholarsrepository.llu.edu/etd/1305
Collection
Loma Linda University Electronic Theses and Dissertations
Collection Website
http://scholarsrepository.llu.edu/etd/
Repository
Loma Linda University. Del E. Webb Memorial Library. University Archives
Included in
Biochemistry Commons, Microbiology Commons, Pathology Commons