Abstract
The assay of Bursa-derived and Thymus-derived lymphocytes (B and T cells) in man has become increasingly useful during the past few years in normal and in clinical conditions. There has been quite a range reported in the literature in the percent of B and T cells in normal subjects by different investigators.
The purpose of this study was to see if monocyte contamination was one cause of non-specific rosette formation. Twenty-one subjects were used to compare the technique involving trypsin and neuraminidase treatment of erythrocytes and the technique of glass wool removal of monocytes. A peroxidase stain was used to confirm monocytes on ten of the twenty-one subjects and a fluorescent antibody stain was done on four subjects to confirm the EAC rosettes as B cells.
There was a definite decrease in the number of monocytes after glass wool column separation. This led to a slight increase in the number of T cells and a slight decrease of B cells.
A method is described which will help eliminate more of the monocytes and give a more accurate value of B and T cells.
LLU Discipline
Microbiology
Department
Microbiology
School
Graduate School
First Advisor
Benjamin H. S. Lau
Second Advisor
Charles E. Winter
Third Advisor
Earl W. Lathrop
Degree Name
Master of Science (MS)
Degree Level
M.S.
Year Degree Awarded
1976
Date (Title Page)
6-1976
Language
English
Library of Congress/MESH Subject Headings
Lymphocytes; B-Lymphocytes; T-Lymphocytes.
Type
Thesis
Page Count
vi; 23
Digital Format
Digital Publisher
Loma Linda University Libraries
Copyright
Author
Usage Rights
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Recommended Citation
Forsythe, William F. Jr., "Comparison of Two Techniques for Enumeration of Human B and T Lymphocytes" (1976). Loma Linda University Electronic Theses, Dissertations & Projects. 1381.
https://scholarsrepository.llu.edu/etd/1381
Collection
Loma Linda University Electronic Theses and Dissertations
Collection Website
http://scholarsrepository.llu.edu/etd/
Repository
Loma Linda University. Del E. Webb Memorial Library. University Archives