The assay of Bursa-derived and Thymus-derived lymphocytes (B and T cells) in man has become increasingly useful during the past few years in normal and in clinical conditions. There has been quite a range reported in the literature in the percent of B and T cells in normal subjects by different investigators.
The purpose of this study was to see if monocyte contamination was one cause of non-specific rosette formation. Twenty-one subjects were used to compare the technique involving trypsin and neuraminidase treatment of erythrocytes and the technique of glass wool removal of monocytes. A peroxidase stain was used to confirm monocytes on ten of the twenty-one subjects and a fluorescent antibody stain was done on four subjects to confirm the EAC rosettes as B cells.
There was a definite decrease in the number of monocytes after glass wool column separation. This led to a slight increase in the number of T cells and a slight decrease of B cells.
A method is described which will help eliminate more of the monocytes and give a more accurate value of B and T cells.
Benjamin H. S. Lau
Charles E. Winter
Earl W. Lathrop
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Lymphocytes; B-Lymphocytes; T-Lymphocytes.
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This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Forsythe, William F. Jr., "Comparison of Two Techniques for Enumeration of Human B and T Lymphocytes" (1976). Loma Linda University Electronic Theses, Dissertations & Projects. 1381.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives