Abstract

The complement fixation test (CF) is currently the test most often used to determine the presence of antibody to human Cytomegalovirus (CMV). Several more recent techniques have now been adapted for this purpose. A comparison of CMV antibody titers was made between the CF test, a commercially available enzyme-linked immunosorbent assay (ELISA), an indirect immunofluorescent technique (IFA), and a modified indirect hemagglutination test (IHA). Forty three serum samples were tested for antibodies to CMV by each of the above procedures. The ELISA, IFA, and IHA titers were in close agreement on all samples tested. The ELISA, IFA, and IHA titers were all equal to or greater than the CF titer for 38 of the 43 samples (88%). Two samples were anticomplementary in the CF test but gave readable results in the other procedures. Two samples with low CF titers were negative in each of the other tests. The CF test was the least sensitive of the procedures examined. The commercial ELISA system appeared to be the most practical method and offered the highest degree of sensitivity in detecting antibodies to CMV.

LLU Discipline

Microbiology

Department

Microbiology

School

James D. Kettering

First Advisor

John E. Lewis

Second Advisor

George M. Lessard

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1982

Date (Title Page)

8-1982

Language

English

Library of Congress/MESH Subject Headings

Cytomegaloviruses; Immunologic Techniques.

Type

Dissertation

Page Count

ii; 24

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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Microbiology Commons

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