Carbon Dioxide Fixation in Schistosoma Mansoni

Author

Clint Earl Carter

Abstract

Adults of the blood fluke Schistosoma mansoni were perfused from the hepatic portal system of CF1 female mice sixty to seventy days post infection. The worms were then incubated in Eagle’s minimal essential medium with glutamate and 0.03 mg/100 ml of nonlabelled aspartic acid. To this medium was added 10 μc of either NaH¹⁴0₃, glucose-UC¹⁴ or aspartate-4-C¹⁴. The worms were found to fix carbon from the NaH¹⁴0₃ and to incorporate the glucose and aspartate. The amount of activity recovered in five different chemical fractions after two hours incubation was determined for each radiochemical used and compared to determine the relative significance of carbon dioxide in Schistosoma mansoni metabolism.

All five fractions which were separated from worms incubated in glucose were significantly different from the control samples. The worms incubated in aspartate and sodium bicarbonate incorporated these compounds into every fraction except the ethanol fraction. Worms incubated in both aspartate and sodium bicarbonate had the greatest amount of incorporation of label in the protein fraction with the least amount in the ethanol soluble fraction.

Under the experimental conditions employed glucose appears to be the most important source of carbon when compared with either aspartate or bicarbonate.

LLU Discipline

Biology

Department

Biology

School

Graduate School

First Advisor

Ariel A. Roth

Second Advisor

Edward D. Wagner

Third Advisor

R. Bruce Wilcox

Degree Name

Master of Arts (MA)

Degree Level

M.A.

Year Degree Awarded

1967

Date (Title Page)

6-1967

Language

English

Library of Congress/MESH Subject Headings

Carbon Dioxide; Schistosoma mansoni

Type

Thesis

Page Count

vi; 16

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Copyright

Author

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Recommended Citation

Carter, Clint Earl, "Carbon Dioxide Fixation in Schistosoma Mansoni" (1967). Loma Linda University Electronic Theses, Dissertations & Projects. 1451.
https://scholarsrepository.llu.edu/etd/1451

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives