Abstract
Cercariac of Schistosoma mansoni were studied for glycogen utilization, uptake of glucose from solution, lactic acid production, and respiration.
Using the anthrone method of determination of carbohydrate, the amount of glycogen used by a single cercaria in 4 hours is determined as 43.2 x 10-4 μg ± 6.6 x 10-4 μg.
Glucose was not observed to have been used from solution under the conditions of the experiment, even in the presence of trehalose. The determinations were made by the Glucostat method.
After deproteinization and desugarization, aliquots of supernatant from cercarial suspensions were analyzed for lactic acid using p-phenylphenol and sulfuric acid. The production of significant amounts of lactic acid by the cercariae was not observed.
Respiration was measured on a Warburg constant volume respirometer. The amount of oxygen used by a single cercaria in 4 hours is determined as 29.0 x 10 -4 μl ± 3.4 x 10-4 μl. The amount of carbon dioxide produced by a single cercaria in 4 hours is determined as 34.6 x 10 -4 μl ± 4.6 x 10 -4 μl. A respiratory quotient of 1.9 ± 0.08 was obtained.
LLU Discipline
Biology
Department
Biology
School
Graduate School
First Advisor
Ariel A. Roth
Second Advisor
Charles E. Winter
Third Advisor
Kenneth A. Arendt
Fourth Advisor
Donald M. Brown
Fifth Advisor
Edward D. Wagner
Degree Name
Master of Arts (MA)
Degree Level
M.A.
Year Degree Awarded
1968
Date (Title Page)
6-1968
Language
English
Library of Congress/MESH Subject Headings
Carbohydrates -- metabolism Schistosoma mansoni.
Type
Thesis
Page Count
v; 24
Digital Format
Digital Publisher
Loma Linda University Libraries
Copyright
Author
Usage Rights
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Recommended Citation
Dunham, A. Eugene Jr., "Some Aspects of Carbohydrate Metabolism in the Cercariae of SCHISTOSOMA MANSONI Sambon, 1907" (1968). Loma Linda University Electronic Theses, Dissertations & Projects. 1461.
https://scholarsrepository.llu.edu/etd/1461
Collection
Loma Linda University Electronic Theses and Dissertations
Collection Website
http://scholarsrepository.llu.edu/etd/
Repository
Loma Linda University. Del E. Webb Memorial Library. University Archives
Included in
Animal Sciences Commons, Biochemical Phenomena, Metabolism, and Nutrition Commons, Biology Commons, Carbohydrates Commons, Microbiology Commons, Parasitology Commons