Abstract
The survival/adaptation of Fillifactor alocis, a fastidious gram positive asaccharolytic anaerobe in the inflammatory environment of the periodontal pocket, requires an ability to overcome oxidative stress. Moreover, emerging observations suggest its pathogenic characteristics are highlighted by its capacity to survive in the oxidative-stress microenvironment of the periodontal pocket and its likely ability to modulate the microbial community dynamics. There is still a significant gap in our understanding of F. alocis mechanism of oxidative stress resistance and its impact on the virulence and pathogenicity of the microbial biofilm. The pathogenicity of F. alocis may be impacted by its response to oxidative stress in the subgingival crevice and understanding this stress response mechanism is key to providing insight in the pathogen’s role in disease onset. This project traverses the current understanding of F. alocis, and primarily focuses on elucidating the role of the FA1654 protein in the oxidative stress machinery of F. alocis. The data obtained indicates that the FA1654 protein may function as a DNA starvation and stationary phase protection protein (DPS), that can modulate Fenton reaction- mediated oxidative stress via iron sequestration.
LLU Discipline
Microbiology and Molecular Genetics
Department
Microbiology
School
School of Medicine
First Advisor
Mark Johnson
Second Advisor
Hansel Fletcher
Third Advisor
So Ran Kwon
Fourth Advisor
Christopher Perry
Fifth Advisor
Ubaldo Soto
Degree Name
Doctor of Philosophy (Medical Science)
Degree Level
Ph.D.
Year Degree Awarded
2023
Date (Title Page)
12-2022
Language
English
Library of Congress/MESH Subject Headings
Bacteria, Anaerobic; Periodontal Pocket; Oxidative Stress
Type
Dissertation
Page Count
xv, 120 p.
Digital Format
Digital Publisher
Loma Linda University Libraries
Copyright
Author
Usage Rights
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Recommended Citation
Mangar, Malissa Makaylia, "The role of the Filifactor alocis hypothetical protein FA1654 in oxidative stress resistance" (2022). Loma Linda University Electronic Theses, Dissertations & Projects. 1762.
https://scholarsrepository.llu.edu/etd/1762
Collection
Loma Linda University Electronic Theses and Dissertations
Collection Website
http://scholarsrepository.llu.edu/etd/
Repository
Loma Linda University. Del E. Webb Memorial Library. University Archives