Abstract

Neuroendocrine mediators have been shown to influence a broad spectrum of immune functions suggesting the existence of a neuroimmune regulatory axis effecting feedback control of the immune response. Most studies in support of this paradigm have employed either intact animals or immune cells derived from blood and other tissues. Under these conditions it becomes difficult to cope with numerous variables associated with the complex milieu of the immune system and the interpretation of data. An in vitro model using a murine macrophage cell line, J774, was developed to circumvent these problems. Using this model, macrophages activated by exposure to the biological response modifiers lipopolysaccharide bacillus Calmettc-Guerin, Corynebacterium parvum, α + ß interferon and γ-interferon exhibited a readily observed and quantitated chemiluminescent oxidative burst. This activity was modulated by the neuropeptides ß-endorphin, dynorphin A, neurotensin and tuftsin as well as the neuroendocrine hormones norepinephrine γ-aminobutyric acid and the pineal hormone melatonin. In the case of norepinephrine, the mechanism of action may involve hormone-mediated hyperpolarization of the macrophage membrane potential.

LLU Discipline

Biology

Department

Biology

School

Graduate School

First Advisor

Benjamin H. S. Lau

Second Advisor

Leonard R. Brand

Third Advisor

A. Graham Maxwell

Fourth Advisor

Elwood S. McCluskey

Fifth Advisor

Robert W. Teel

Degree Name

Doctor of Philosophy (PhD)

Degree Level

Ph.D.

Year Degree Awarded

1989

Date (Title Page)

6-1989

Language

English

Library of Congress/MESH Subject Headings

Neuroendocrinology; Immune System; Hormones; Luminescence; Macrophages

Type

Dissertation

Page Count

vii; 75; 2

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

Included in

Biology Commons

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