Abstract
Neuroendocrine mediators have been shown to influence a broad spectrum of immune functions suggesting the existence of a neuroimmune regulatory axis effecting feedback control of the immune response. Most studies in support of this paradigm have employed either intact animals or immune cells derived from blood and other tissues. Under these conditions it becomes difficult to cope with numerous variables associated with the complex milieu of the immune system and the interpretation of data. An in vitro model using a murine macrophage cell line, J774, was developed to circumvent these problems. Using this model, macrophages activated by exposure to the biological response modifiers lipopolysaccharide bacillus Calmettc-Guerin, Corynebacterium parvum, α + ß interferon and γ-interferon exhibited a readily observed and quantitated chemiluminescent oxidative burst. This activity was modulated by the neuropeptides ß-endorphin, dynorphin A, neurotensin and tuftsin as well as the neuroendocrine hormones norepinephrine γ-aminobutyric acid and the pineal hormone melatonin. In the case of norepinephrine, the mechanism of action may involve hormone-mediated hyperpolarization of the macrophage membrane potential.
LLU Discipline
Biology
Department
Biology
School
Graduate School
First Advisor
Benjamin H. S. Lau
Second Advisor
Leonard R. Brand
Third Advisor
A. Graham Maxwell
Fourth Advisor
Elwood S. McCluskey
Fifth Advisor
Robert W. Teel
Degree Name
Doctor of Philosophy (PhD)
Degree Level
Ph.D.
Year Degree Awarded
1989
Date (Title Page)
6-1989
Language
English
Library of Congress/MESH Subject Headings
Neuroendocrinology; Immune System; Hormones; Luminescence; Macrophages
Type
Dissertation
Page Count
vii; 75; 2
Digital Format
Digital Publisher
Loma Linda University Libraries
Copyright
Author
Usage Rights
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Recommended Citation
Tosk, Jeffrey M., "Modulation of Macrophage Chemiluminescence by Biological Response Modifiers and Neuroendocrine Hormones" (1989). Loma Linda University Electronic Theses, Dissertations & Projects. 1912.
https://scholarsrepository.llu.edu/etd/1912
Collection
Loma Linda University Electronic Theses and Dissertations
Collection Website
http://scholarsrepository.llu.edu/etd/
Repository
Loma Linda University. Del E. Webb Memorial Library. University Archives