Abstract

A study was undertaken to determine the concentration at which a sealer reaches a point of nontoxicity. An in vitro model utilizing HeLa cells and human fibroblasts was used to observe the effect of various concentrations of eight freshly mixed and eight set root canal sealers. Evaluation of toxicity was determined by cell survival which was measured by incorporation of 3H-thymidine into the nucleic acid components of each cell type used. Each dilution tested was allowed direct contact with the cells for 24 hours before toxicity evaluation. The results indicate that all sealers at concentrations presently used are cytotoxic in both a fresh and set state. It was also observed that as a sealer is diluted its toxicity decreases. There appeared to be an increase in the sensitivity of fibroblasts to the sealer dilutions being evaluated as compared to the HeLa cells.

Department

Dentistry

School

Graduate School

First Advisor

Mahmoud Torabinejad

Second Advisor

Leif K. Bakland

Third Advisor

Jan Egelberg

Fourth Advisor

James Kettering

Fifth Advisor

Grenith J. Zimmerman

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1980

Date (Title Page)

6-1980

Language

English

Library of Congress/MESH Subject Headings

Root Canal Filling Materials

Type

Thesis

Page Count

vii; 66

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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