Abstract

Previous studies have evaluated the effects of various hemostatic agents on radicular tissues following apical surgery. The purpose of this study was to examine the early osseous responses to seven hemostatic agents under identical conditions in rabbits. Sixteen adult male New Zealand white rabbits were used for this study. Following anesthesia, utilizing a midline submandibular incision, standardized bony defects were prepared on the lateral-superior regions of the mandible. Test materials were placed using standardized volumes and exposure times. Bone wax, Surgicel, Capset, alginate, Cut-Trol, Hemogin-L, and silver nitrate were compared to control sites irrigated with saline. Test materials or controls were assigned to cavities utilizing a cyclic shift design. Following the exposure, the bony defects were curetted and then irrigated with saline prior to suturing. Animals were sacrificed ninety-six (96) hours after exposure. Mandibular segments including the experimental sites were prepared and evaluated histologically utilizing H&E and TRAP staining. Using Image-Pro Plus software, fibroblast-like cell density, clot resolution, osteoclast activity, and the extent of bone necrosis were analyzed. The Kruskal-Wallis rank test, Wilcoxon-Mann-Whitney (WMW) test and the Two-Sample Binomial test were utilized. Pair-wise analysis (WMW) showed only silver nitrate had significantly lower fibroblast-like cell densities compared to saline (p < 0.0001) and compared to all other experimental materials (p < 0.001). Residual clots were not significantly different for the eight materials (p = 0.624). Osteoclast activity in silver nitrate sites was significantly lower than for all other materials (p < 0.038). Significantly more bone necrosis occurred in samples treated with Surgicel, Capset, alginate, Cut-Trol, Hemogin-L and silver nitrate compared to saline (p = 0.0003). No significant difference was found between samples treated with bone wax and those treated with saline (p = 0.131). Based on the results of this study, it appears that only silver nitrate significantly reduced fibroblast-like cell densities. Most hemostatic agents evaluated created mild osseous necrosis. Bone wax caused the least amount of bone necrosis.

Department

Dentistry

School

Graduate School

First Advisor

Mahmoud Torabinejad

Second Advisor

Paul McMillan

Third Advisor

Shahrokh Shabahang

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2003

Date (Title Page)

9-2003

Language

English

Library of Congress/MESH Subject Headings

Hemostatic Techniques; Hemostatis, Surgical; Mandible -- surgery

Type

Thesis

Page Count

viii; 57

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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