Abstract

The urinary excretion of 3-Methylhistidine was used as an index of muscle catabolism in four groups of obese persons receiving a Protein Sparing Modified Fast (PSMF). The composition of the supplements were as follows: Group I: 75 g protein, 25 g complex carbohydrate (starch) per day; Group II: 25 g protein, 75 g complex carbohydrate (starch) per day; Group III: 75 g protein, 25 g simple carbohydrate (glucose) per day; Group IV: 25 g protein, 75 g simple carbohydrate (glucose) per day. The PSMF lasted for seven days and a 24-hour urine sample was collected at the beginning of the fast and at the end for analysis of 3-Methylhistidine (3-MeHis). A fluorescent derivative was prepared from 3-MeHis and determined by High Pressure Liquid Chromatography (HPLC) using UV absorption at 254 nm.

There were no significant differences between groups in muscle catabolism when analyzed by group, or by sex and group, or by combining high protein groups I and III and comparing to low protein groups II and IV.

Twenty-five grams carbohydrate appears to be sufficient to supply glucose needs during fasting, and that type of carbohydrate may have little to do with protein sparing at the levels used in this study.

Department

Nutrition

School

Graduate School

First Advisor

U. D. Register

Second Advisor

Ella Haddad

Third Advisor

James Blankenship

Fourth Advisor

Kenneth Burke

Fifth Advisor

Grenith Zimmerman

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1981

Date (Title Page)

8-1981

Language

English

Library of Congress/MESH Subject Headings

Fasting; Muscle Proteins; Dietary Proteins

Type

Thesis

Page Count

v; 26

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

Download

Share

COinS