Reagan Ondler

Abstract

The purpose of this study was to determine the effect of insulin and fluoride on dentinal fluid movement (FM) in high-sucrose fed rats as mediated through the hypothalamic-parotid gland endocrine axis. In conjunction with the fluoride study, the possible involvement of cAMP was examined through the use of several methyl xanthines which are recognized as phosphodiesterase inhibitors.

Twenty-one day old, male Sprague Dawley rats were maintained on a high-sugar diet (62% sucrose) for 8-9 days at which time intravenous or arterial infusions of insulin, fluoride or the methyl xanthines were performed. Additional procedures included collecting plasma from rats which had been infused with insulin, fluoride or the methyl xanthines, and infusing it into other high-sugar fed rats.

Infusion of insulin resulted in the stimulation of dentinal FM. Plasma assays showed an increase in FM activity in the blood after insulin infusion, suggesting the possibility of stimulation through the hypothalamic-parotid gland endocrine axis.

Intravenous infusion of fluoride stimulated the increase of dentinal FM in sugar-fed rats. Plasma assays showed the presence of a fluid moving principle in the blood as a result of fluoride infusion. Parotidectomized rats which were infused intravenously with fluoride showed no increase in dentinal FM indicating that the parotid gland was required for its stimulatory effect.

The infusion of methyl xanthines resulted in an increase of dentinal FM similar to that observed with fluoride which indicates the possibility that fluoride and methyl xanthines may exercise their effect on FM through the hypothalamic-parotid gland endocrine axis utilizing the cAMP stimulating mechanism.

LLU Discipline

Physiology

Department

Physiology

School

Graduate School

First Advisor

John Leonora

Second Advisor

Ralph R. Steinman

Third Advisor

Kenneth R. Arendt

Fourth Advisor

Elwood S. McCluskey

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1975

Date (Title Page)

6-1975

Language

English

Library of Congress/MESH Subject Headings

Cariostatic Agents -- physiology; Insulin; Fluorides

Type

Thesis

Page Count

vi; 46

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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