Abstract

This study evaluated candidate carriers for recombinant human bone morphogenic protein-2 (rhBMP-2) in periodontal reconstructive surgery.

Canine demineralized bone matrix (DBM), bovine inorganic crystallene bone matrix (Bio-Oss), bovine derived microfibrillar collagen matrix (Helistat), poly (D,L-lactide-coglycolide) micropaiticles (PLGA), and polylactic acid granules (Drilac) were used with rhBMP-2 (20 (µg/100 µl implant volume) in routine critical size canine supraalveolar periodontal defects. Contralateral defects in six beagle dogs were randomly assigned to receive: DBM/rhBMP-2, DBM-control, Bio-Oss/rhBMP-2, Helistat/rhBMP-2, PLGA/rhBMP-2, or Drilac/rhBMP-2, all with autologous blood except for Helistat and PLGA. Animals were sacrificed eight weeks post-surgery and block sections of the defects were processed for light microscopy. Histometric analysis included: defect height, connective tissue repair, cementum height, bone height, bone area, bone density, root resorption, and ankylosis. Mean and standard deviation values for each parameter were calculated for each carrier in each of two dogs, as well as mean values for both animals. Notable differences were evident among the candidate carriers examined, particularly with respect to cementum and bone regeneration. The results suggest that bovine derived bone matrix and canine demineralized bone matrix provide the best support for rhBMP-2 driven periodontal regeneration in the dog.

LLU Discipline

Periodontics

Department

Periodontics

School

Graduate School

First Advisor

Ulf M. E. Wikesjö

Second Advisor

Thorarinn J. Sigurdsson

Third Advisor

Paul J. McMillan

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1995

Date (Title Page)

6-1995

Language

English

Library of Congress/MESH Subject Headings

Dogs; Periodontics -- surgery

Type

Thesis

Page Count

vii; 36

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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