Abstract

The purpose of this investigation was to determine the antimicrobial effect of Biopure MTAD (a mixture of a tetracycline isomer, an acid, and a detergent) as a final irrigant on eight strains of Enterococcus faecalis (E. faecalis) in contaminated root canals of extracted human teeth. In addition, the Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) of MTAD were also measured on the same strains of bacteria. Two-hundred and forty extracted human teeth were instrumented using 1.3% NaOCl followed by 17% EDTA to remove the smear layer. The teeth were randomly contaminated with one of eight strains of E. faecalis for 4 weeks. After using 1.3% NaOCl as a root canal irrigant, the root canal and the external surface of each tooth were exposed to a 5-min application of MTAD. Teeth or dentin shavings were cultured to determine presence or absence of the test bacteria. MTAD was effective in completely eliminating growth in seven out of eight strains of E. faecalis. The results of the MIC/MLC tests showed that MTAD inhibited E. faecalis growth when diluted 1:2048 times in two strains and 1:8192 times in six strains. MTAD killed most strains of E. faecalis when diluted 1:512 times with a range from 1:128 to 1:1024. The results of these experiments show that MTAD is an effective irrigant against most strains of E. faecalis.

Department

Dentistry

School

Graduate Studies

First Advisor

Mahmound Torabinejad

Second Advisor

Neal Johnson

Third Advisor

Shahrokh Shabahang

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2007

Date (Title Page)

8-2007

Language

English

Library of Congress/MESH Subject Headings

Enterococcus Faecalis -- drug effects; Root Canal Therapy; Microbial Sensitivity Tests

Type

Thesis

Page Count

ix; 62

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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