Abstract

For over thirty years now, research has been carried out to isolate and purify bone morphogenetic protein (BMP), a substance which has been shown to induce heterotopic bone formation in various animal species. Recent advances in the fields of developmental biology, molecular biology and genetics, have shown that the BMPs not only are responsible for postfetal bone induction (including normal bone remodeling, healing and repair), but are also critical during embryogenesis - not only in regards to the skeletal system, but quite possibly in the morphogenesis and pattern formation of other tissues and organs as well.

Due to their bone induction capabilities, the BMPs have been studied for use in situations of trauma or disease necessitating bone replacement therapy. Periodontal disease is one such entity, resulting in bone as well as attachment loss around teeth. This study evaluated bone and cementum regeneration following periodontal reconstructive surgery using recombinant human bone morphogenetic protein-2 (rhBMP-2) applied with poly (D,L-lactide-co-glycolide) (PLGA) microparticles and autologous blood in six beagle dogs. Critical size mandibular supraalveolar periodontal defects were created, with contralateral quadrants receiving rhBMP-2 or control vehicle. The animals were sacrificed eight weeks postsurgery, and block sections processed for histometric analysis. The defect height, height and area of bone regeneration, cementum regeneration, root resorption, and ankylosis were recorded, and group means and standard deviations analyzed (Student t test; n=6). Statistically significant differences were obtained between groups for both height and area of bone regeneration, cementum regeneration, and root resorption. These results suggest that rhBMP-2 not only induces extensive bone regeneration in this model, but may also facilitate conditions which enhance cementum formation as well.

LLU Discipline

Periodontics

Department

Periodontics

School

Graduate School

First Advisor

Ulf M. E. Wikesjö

Second Advisor

Thorarinn J. Sigurdsson

Third Advisor

Paul J. McMillian

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1996

Date (Title Page)

6-1996

Language

English

Library of Congress/MESH Subject Headings

Periodontics; Periodontal Diseases -- surgery; Morphogenesis; Transforming Growth Factor beta

Type

Thesis

Page Count

2 vii; 62

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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