Abstract

Children often present with dental trauma to pediatric emergency departments and pediatric dental offices. There is a wide spectrum of types of dental trauma. Although research has been done concerning the treatment of avulsed teeth, the current recommendations in the published guidelines might still be improved for better long term prognoses. One may speculate that ultrasonification of an avulsed tooth prior to replantation may dislodge existing surface bacteria and improve the prognosis for the tooth. This was as an in vitro study to test this concept. Epithelial cells were grown in 6-well tissue culture plates. Standardized amounts of Staphylococcus aureus were introduced to each well of cells, and the cells were then incubated for 30 minutes at 37° C, rinsed, placed in an ultrasonic bath for various periods of time, and then rerinsed. Finally, all bacterial samples were plated to be quantified. The results were statistically analyzed utilizing the Mann-Whitney U-test. The results revealed no statistically significant difference between the number of dislodged bacteria in the rinsed-only control samples and the ultrasonified samples. Extracted teeth were also used in this study to test the effect of the ultrasonification on the dislodgement of surface bacteria when compared to manual saline rinsing. Results revealed no significant difference between ultrasonification and manual rinsing on the dislodgement of surface bacteria from extracted teeth. It appears from this study, that ultrasonification has no advantage over manual rinsing in dislodging bacteria from the surface of cells of avulsed teeth.

LLU Discipline

Pediatric Dentistry

Department

Pediatric Dentistry

School

Graduate School

First Advisor

John Peterson

Second Advisor

Leif K. Bakland

Third Advisor

James Kettering

Fourth Advisor

Jay Kim

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2003

Date (Title Page)

6-2003

Language

English

Library of Congress/MESH Subject Headings

Pediatric Dentistry; Epithelial Cells; Toothavulsion; Staphylococcus Aureus; Ultrasonic Therapy; Tooth Resorption; Tooth Replantation -- methods; Comparative Study

Type

Thesis

Page Count

xi; 32

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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