Abstract

The field of dentistry uses the debriding properties of ultrasonic vibration. It is unknown if this property is detrimental to the periodontal ligament (PDL) of an avulsed tooth. This information is important when a clinician is faced with a debris-covered avulsed tooth following a traumatic event. The purpose of this study was to determine the effect that ultrasonic vibration has on those PDL cells most numerous and most vital to a successful tooth replantation, namely, the fibroblasts.

Several fibroblast cell sources were exposed to varying ultrasonic times. These included a commercially available cell line of human foreskin fibroblast (HFF), a harvested and cultured human periodontal ligament fibroblast (HPDLF) cell line and PDL fibroblast cells bound to the root surfaces of freshly extracted teeth. The HFF cells were exposed to ultrasonic vibration between 2-45 minutes. The HPDLF cells and the bound PDL fibroblasts were exposed to 5, 10 and 15 minutes of ultrasonic vibration. The latter groups were also compared to similar groups of PDL bound cells that were dried for 30 and 60 minutes prior to ultrasonification. This was to determine a difference in cell survival with ultrasonics when cells were dried. The vitality of the harvested fibroblast cells was assessed by the ability to form a monolayer. Growth indicated survival of the cell lineage and possibly a favorable replantation prognosis.

The results of this study indicated that fibroblast cell lines from both HFF and HPDL were not adversely affected by ultrasonics up to 45 minutes and 15 minutes respectively. Fibroblasts harvested from the PDL of freshly extracted teeth following 5, 10 and 15 minutes of ultrasonic were not affected. However, a dry time of 30 minutes significantly decreased cell vitality (p < 0.0001) and a dry time of 60 minutes resulted in complete cell death (p < 0.0001).

Ultrasonification of teeth up to 15 minutes did not adversely affect fibroblast vitality. Extra-oral dry time of 30-60 minutes appeared to be the most destructive to cell vitality, regardless of ultrasonic vibration. In conclusion, this study showed that the ultrasonic cleaner may have little or no detrimental effect on the root surface fibroblasts when used for tooth debridement prior to replantation.

LLU Discipline

Pediatric Dentistry

Department

Pediatric Dentistry

School

Graduate School

First Advisor

John Peterson

Second Advisor

Leif K. Bakland

Third Advisor

James Kettering

Fourth Advisor

Jay Kim

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

2003

Date (Title Page)

6-2003

Language

English

Library of Congress/MESH Subject Headings

Ultrasonics; Periodontal Ligament -- injuries; Tooth Replantation; Fibroblasts -- cytology -- metabolism; Vibration -- adverse effects; Periodontal Ligament -- growth & development; Tooth Avulsion -- therapy

Type

Thesis

Page Count

x; 36

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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