Abstract

Animal models are frequently consulted for histometric analysis of reconstructive periodontal therapy. Statistical analysis of induced lesion defects and surgically created defects from our laboratory suggest defect size homogeneity is critical for the model to discriminate treatment effects. This study characterizes natural disease defects in beagle dogs. Buccal-lingual histologic sections from the 2nd, 3rd, and 4th mandibular premolar teeth (P2, P3, P4) from 6 aged beagle dogs with natural disease defects were evaluated for variation in defect height between pair matched tooth types, and jaw quadrants, as well as estimated confidence intervals for treatment differences between left and right pair matched teeth and quadrants. Mean defect height ranged from 2.3±0.09 mm for right P2 to 4.1±1.6 mm for right P4. Using confidence intervals for the mean difference between jaw quadrants, it was determined that the mean difference could be as large as 0.8,1.7 and 3.0 mm for P2, P3 and P4, respectively. With the apparent variation in defect baseline, it is suggested that the natural disease model has limited merit in determining treatment effects following reconstructive periodontal therapy.

The effect of a wound stabilizing ePTFE membrane was evaluated in acute supraalveolar periodontal defects in mandibular premolar teeth conditioned with heparin. Heparin has been demonstrated to compromise connective tissue repair to the root surface and promote long junctional epithelium formation in this model. Membrane treated teeth demonstrated near complete connective tissue repair (98% of defect height) compared to 86% for controls (P < 0.05), as well as enhanced bone regeneration (P < 0.05). Bone regeneration was strongly correlated with area under the membrane (r2=0.993; P=0.002), yet poorly correlated with height of the membrane related to the defect height (r2=0.209; P=0.439). These results suggest that ePTFE membranes support wound stabilization and enhance bone regeneration.

LLU Discipline

Periodontics

Department

Periodontics

School

Graduate School

First Advisor

Ulf M.E. Wikesjö

Second Advisor

Grenith J. Zimmerman

Third Advisor

Paul J. McMillan

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1994

Date (Title Page)

6-1994

Language

English

Library of Congress/MESH Subject Headings

Bone Regeneration; Periodontal Diseases -- therapy; Wound Healing; Membranes, Artificial; Dogs

Type

Dissertation

Page Count

2 viii; 36

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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