Abstract

In comparing the growth of polyoma virus in secondary cell cultures of a mouse lymphoma (P388D1, a known polyoma-supporting strain) and normal mouse fibroblasts (Earle’s L cells), the following observations have been made.

The L cell is capable of supporting the growth of the polyoma virus with reproduction and release of infectious particles. Viruses appear in the fluid from time to time over a period of several weeks. This probably represents a carrier state of the virus in L cells. Many of the L cells in infected cultures are not killed by the virus infection.

Virus particles released by L cells and by P388D1 cells represent hemagglutinating, infectious units capable of further reproduction in suitable animal or tissue culture cells hosts.

In single cycles polyoma growth in L cells and P388D1 cells, assayed by the mouse antibody production test, adsorption of the L cells was affected over a longer period of time than the P388D1 cells perhaps representing a less efficient system of penetration by the virus. Cell associated virus in both cell lines undergoes a steady increase from the end of the first day until the third day, at which time it begins to decline. Free virus declines during the early hours of infection, but increases consistently approximately 36 hours until it reaches a maximum, in 7 days, of 100- to 200-fold increase of total virus.

Cytochemical and fluorescent antibody staining revealed evidence of virus intracellularly. Fluorescent antibody labelling of particles in the nuclei and occasionally the cytoplasm have identified the virus as polyoma. Characteristic polyoma cytopathogenicity in the P388D1 cells was consistent with the findings of other workers. A more general cytopathogenicity was found in heavily infected L cells, with less multiplication of the cells in the infected cultures than in the controls.

LLU Discipline

Microbiology

Department

Microbiology

School

Graduate Studies

First Advisor

Robert L. Nutter

Second Advisor

Charles E. Winter

Third Advisor

Jack D. Zwemer

Fourth Advisor

U. D. Register

Fifth Advisor

Carrol S. Small

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1961

Date (Title Page)

6-1961

Language

English

Library of Congress/MESH Subject Headings

Polyomaviruses

Type

Dissertation

Page Count

ix; 79

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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