Abstract

Cell mediated immune (CMI) cytotoxic reactivity of Balb/c mice against H238 cells, a Herpes simplex virus (HSV) Type 2 - induced sarcoma, was measured by the 125IUdR release assay. The Balb/c mouse response to the growing HSV-induced tumor, treated with radiation and , was measured by survival rate, tumor growth and immune cytotoxicity of spleen and peritoneal exudate cells (PEC) as determined by 125IUdR release assay. Subcutaneous (s.c.) inoculation of 1 x 106 H238 cells (high dose) produced progressive tumor growth while s.c. inoculation of 1 x 104 H238 cells (low dose) produced no tumors. A kinetic study was made of the primary and secondary CMI response. These responses were found to be biphasic with early (days 2-8 after inoculation) and late (days 18-20) components. The primary early response of the high dose inoculum was found to precede by four days the CMI response of the low dose [inculum], while the late response of low dose was greater in magnitude and duration than that of the high dose. For the secondary CMI response the early component occurred at the same time interval as that of the high dose inoculum, while the late component developed similar to that of the low dose inoculum in magnitude and duration. But as with the 1 x 106 cell inoculum no ability to stop tumor progression developed.

C. parvum, 350 mg, and radiation, 0-3500 rads, in varied combinations were used as therapeutic agents to help the mice destroy the tumor. The C. parvum in combination with radiation increased the survival rate of the mice bearing tumors. The best results were obtained when C. parvum treatment was combined with 800 rads of radiation. This group demonstrated a 53% increase in survival as compared to the radiation treated group without C. parvum. However, overall the combined therapy had no consistent effect on the CMI or PEC's ability to respond to the tumor. Radiation alone had a marked effect on the growth rate of the tumor, causing reduction in tumor size after being administered. 3500 rads, in some cases, caused complete regression of the tumor.

LLU Discipline

Microbiology

Department

Microbiology

School

Graduate School

First Advisor

Robert L. Nutter

Second Advisor

Yuk Lin Ho

Third Advisor

David J. Gusseck

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1979

Date (Title Page)

5-1979

Language

English

Library of Congress/MESH Subject Headings

Neoplasms -- therapy; Corynebacterium; Immunization

Type

Thesis

Page Count

viii; 42

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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