Abstract

A group of 48 enteric bacteria was selected from a total of 170 at Loma Linda University Medical Center because of resistance to three or more antibiotics. They were examined to see if any of the antibiotic resistances were transferred by conjugation to either of two recipient bacteria. These were a restrictionless, antibiotic sensitive, azide resistant mutant of Escherichia coli K 12 designated E. coli 1228 and a similar phenotypic strain of Salmonella typhimurium LT2 designated LB 5000. Forty-three transferred one or more antibiotic resistances to either or both recipients, a frequency of 25.3% of the total bacteria and 89.6% of the selected bacteria.

By following an extraction procedure a selection of strains transferring resistance were found to possess from one to four plasmids per cell with molecular weights ranging from 9.5 kb to 310 kb. The R factors were shown to belong to a variety of incompatibility groups.

The significance of the use of two different recipients is discussed.

LLU Discipline

Microbiology

Department

Microbiology

School

Graduate School

First Advisor

Leonard R. Bullas

Second Advisor

John E. Lewis

Third Advisor

George Lessard

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1985

Date (Title Page)

6-1985

Language

English

Library of Congress/MESH Subject Headings

Escherichia coli; Salmonella typhimurium; Drug Resistance, Microbial

Type

Thesis

Page Count

v; 73

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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