Abstract

Experimental female rats were given daily injections of synthetic estrogens for six months. The effect on rat ovarian 20α-OH-SDH was not definitive. Diethylstilbestrol and 17α-ethynylestradiol caused a significant drop in activity as compared with controls while mestranol caused a slight increase in activity. A synthetic progestin, medroxyprogesterone, had no apparent effect on the activity of the enzyme.

Rat ovarian 20α-OH-SDH was purified fivefold by ammonium sulfate fractionation and chromatography on DEAE-cellulose.

The homogeneity of the enzyme was checked by means of disc gel electrophoresis. At pH 8.2 no significant contamination appeared in the electrophoretic pattern, but at pH 9.0 five distinct bands appeared in the pattern. The irregularity of the pattern indicated that the additional bands were due to impurities rather than to different forms of the enzyme.

Thin-layer gel chromatography was used to obtain an estimate of the molecular weight of the enzyme. Human hemoglobin and bovine serum albumin were used as standards. The molecular weight of the enzyme was estimated at 75,000.

The stereospecificity of 20α-OH-SDH with respect to TPN was determined. Tritium on the A side of TPNH was stoichiometrically transferred to progesterone when the reduction of progesterone was catalyzed by 20α-OH-SDH. Tritium on the B side of TPNH was stoichiometrically retained on the TPN molecule under the same conditions. These data indicate that 20α-OH-SDH is a Class A dehydrogenase.

LLU Discipline

Biochemistry

Department

Biochemistry

School

Graduate School

First Advisor

Raymond A. Mortensen

Second Advisor

Richard E. Beltz

Third Advisor

Donovan A. Courville

Fourth Advisor

Donald W. Hessel

Fifth Advisor

Ulma D. Register

Sixth Advisor

Walter E. Roop

Seventh Advisor

R. Bruce Wilcox

Degree Name

Doctor of Philosophy (PhD)

Degree Level

Ph.D.

Year Degree Awarded

1968

Date (Title Page)

8-1968

Language

English

Library of Congress/MESH Subject Headings

Progesterone -- analysis; Hydrogen

Type

Dissertation

Page Count

ix; 98

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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