Thymic Peptide Modulates Glutathione Redox Cycle and Antioxidant Enzymes in Macrophages

Author

Choon Sil Park

Abstract

The effect of a 6-kDa thymic peptide (TP) on the oxidative burst of a murine macrophage cell line J774 was determined. TP (12.5-200 μg/ml) was incubated with 5 x 10⁵ J774 cells at 37° C and 5% C0₂ for 18 h and oxidative burst was triggered by zymosan; chemiluminescence was amplified by luminol and measured in an automated luminometer. TP exhibited a concentration-dependent inhibition of oxidative burst. To study the mechanisms involved in TP’s inhibition of oxidative burst, its effect on the glutathione (GSH) redox cycle, GSH biosynthesis, and antioxidant enzymes was investigated. TP was shown to increase the levels of GSH, GSH-peroxidase, and GSH-reductase, indicating its ability to modulate the GSH redox cycle. The activity of antioxidant enzyme superoxide dismutase was enhanced significantly by TP treatment while catalase activity remained unaffected. These results suggest that TP possesses an antioxidant property and may thus be involved in the regulation of free radical mediated reactions.

Key Words: free radicals, chemiluminescence, glutathione, glutathione-reductase, glutathione-peroxidase, superoxide dismutase.

LLU Discipline

Microbiology

Department

Microbiology

School

Graduate School

First Advisor

Benjamin H.S. Lau

Second Advisor

Daila S. Gridley

Third Advisor

Mark S. Johnson

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1993

Date (Title Page)

6-1993

Language

English

Library of Congress/MESH Subject Headings

Macrophages -- physiology; Glutathone -- metabolism; Chemiluminescence; Thymic Factor, Circulating; Superoxide Dismutase

Type

Thesis

Page Count

iv; 23

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Copyright

Author

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Recommended Citation

Park, Choon Sil, "Thymic Peptide Modulates Glutathione Redox Cycle and Antioxidant Enzymes in Macrophages" (1993). Loma Linda University Electronic Theses, Dissertations & Projects. 2618.
https://scholarsrepository.llu.edu/etd/2618

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives