Abstract

Infiltration to an injury site or area of bacterial contamination by polymorphonuclear leucocytes (or neutrophils) is an early and essential phase of the early inflammatory reaction. Their action on foreign matter is the most critical step taken by body defense mechanisms in the first 24 hours. Anything which interferes with that action, phagocytosis, greatly hampers early inflammation. This study was designed to see the effect of elevated glucose levels on neutrophil phagocytosis in a unique strain of genetically diabetic mice.

Twenty-two diabetic and twenty-four control female mice were used. Blood glucose determinations and total white counts were made on each mouse. Staphylococcus epidermidis was added in vitro to the extracted blood of each mouse, incubated at 37°C for 30 minutes and slides were prepared with Wright's stain. A phagocytic index was made from the slides. The mean values of each criteria were determined and compared by "t" test and regression analysis.

When comparing the experimental and control groups, there were highly significant differences in phagocytic index, glucose level, and total white count. The groups were so widely separated on a scattergram, however, it was apparent that they could not be correlated. Regression analysis confirmed it.

The phagocytic indices were much lower and glucose levels and total white counts much higher in the experimental animals than the controls. There was no middle ground. Although elevated glucose levels seem to have a suppressing effect on neutrophil phagocytosis. the effect at any particular glucose concentration could not be determined by this method of analysis.

LLU Discipline

Periodontics

Department

Periodontics

School

Graduate School

First Advisor

Donald F. Adams

Second Advisor

Elmer E. Kelln

Third Advisor

Richard C. Oliver

Fourth Advisor

W. Eugene Rathbun

Degree Name

Master of Science (MS)

Year Degree Awarded

1971

Date (Title Page)

5-1971

Language

English

Library of Congress/MESH Subject Headings

Phagocytosis; Glucose

Type

Thesis

Page Count

v; 19

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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