Abstract
Insulin-like growth factor binding protein-5 (IGFBP-5) is produced by osteoblasts and potentiates IGF mitogenic actions in osteoblast cultures. Progesterone (PG) increases osteoblast proliferation and increases osteoblast IGFBP-5 expression. Mechanisms underlying PG induction of IGFI3P-5 expression were investigated in U2 human osteosarcoma cells. In nuclear run-on analyses, PG increased IGF)3P-5 gene transcription to 400% of control. To define the underlying mechanisms, the human IGF13P-5 gene proximal promoter was cloned and sequenced. The proximal 500 bp of this region contains TATA and CAAT boxes, five putative PG response element (PRE) half-sites and two tandem CACCC boxes. Chloramphenicol acetyltransferase (CAT) reporter constructs containing up to 753 bp of the human IGFBP-5 gene 5'-flanking sequence were made. In U2 cells transfected with reporter construct pCAT753 containing positions -753 to t23, PG increased CAT activity to 134% of untreated control activity (P
LLU Discipline
Biochemistry
Department
Biochemistry
School
Graduate School
First Advisor
Thomas A. Linkhart
Second Advisor
Donna D. Strong
Third Advisor
Subburaman Mohan
Fourth Advisor
R. Bruce Wilcox
Fifth Advisor
Aladar A. Szalay
Sixth Advisor
John F. Sands
Degree Name
Doctor of Philosophy (PhD)
Degree Level
Ph.D.
Year Degree Awarded
1996
Date (Title Page)
6-1996
Language
English
Library of Congress/MESH Subject Headings
Progesterone; Insulin-Like Growth-Factor-Binding-Protein 5; Reverse Transcriptions; Osteoblasts
Type
Dissertation
Page Count
xii; 175
Digital Format
Digital Publisher
Loma Linda University Libraries
Copyright
Author
Usage Rights
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
Recommended Citation
Boonyaratanakornicit, Viroj, "Progesterone Regulation of Insulin like-Growth Factor : Binding Protein-5 Gene Transcription in Human Osteoblasts" (1996). Loma Linda University Electronic Theses, Dissertations & Projects. 658.
https://scholarsrepository.llu.edu/etd/658
Collection
Loma Linda University Electronic Theses and Dissertations
Collection Website
http://scholarsrepository.llu.edu/etd/
Repository
Loma Linda University. Del E. Webb Memorial Library. University Archives