Abstract

Portions of an acetone-hexane (1:4) extract of Ponderosa pine sawdust were exposed to gamma and ultraviolet radiation. The three solutions, including the original, were checked for antibiotic activity on: Staphylococcus aureus Escherichia coli and Bacillus cereus. Results indicated that each solution inhibited the growth of the bacteria.

The three solutions were separated into fractions by 'column' chromatography on silica gel, using chloroform-carbon tetrachloride (1:1) as the elutant and then 10-20-50-80% ethanol in the chloroform- carbon tetrachloride mixture and finally 100% ethanol. Ten fractions were obtained from each sample and these were each tested on the bacteria. Results indicated a number of fractions having antibiotic activity. The original and gamma irradiated sample being about equal in activity, with the ultraviolet irradiated sample showing less activity than the other two. The paper includes I R spectrograms of the most active fractions. They appear to be made up, mainly, of aliphatic acids and aldehydes.

Department

Chemistry

School

Graduate School

First Advisor

William D. Leech

Second Advisor

Maurice E. Mathisen

Third Advisor

George J. Nelson

Fourth Advisor

Bernard E. Tilton

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1967

Date (Title Page)

8-1967

Language

English

Library of Congress/MESH Subject Headings

Ponderosa pine -- Analysis; Wood waste -- Analysis

Type

Thesis

Page Count

vi; 29

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

Included in

Chemistry Commons

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