Portions of an acetone-hexane (1:4) extract of Ponderosa pine sawdust were exposed to gamma and ultraviolet radiation. The three solutions, including the original, were checked for antibiotic activity on: Staphylococcus aureus Escherichia coli and Bacillus cereus. Results indicated that each solution inhibited the growth of the bacteria.
The three solutions were separated into fractions by 'column' chromatography on silica gel, using chloroform-carbon tetrachloride (1:1) as the elutant and then 10-20-50-80% ethanol in the chloroform- carbon tetrachloride mixture and finally 100% ethanol. Ten fractions were obtained from each sample and these were each tested on the bacteria. Results indicated a number of fractions having antibiotic activity. The original and gamma irradiated sample being about equal in activity, with the ultraviolet irradiated sample showing less activity than the other two. The paper includes I R spectrograms of the most active fractions. They appear to be made up, mainly, of aliphatic acids and aldehydes.
William D. Leech
Maurice E. Mathisen
George J. Nelson
Bernard E. Tilton
Master of Science (MS)
Year Degree Awarded
Date (Title Page)
Library of Congress/MESH Subject Headings
Ponderosa pine -- Analysis; Wood waste -- Analysis
Loma Linda University Libraries
This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.
York, Mervin B., "Radiolysis of the Extract from the Sawdust of the Ponderosa Pine" (1967). Loma Linda University Electronic Theses, Dissertations & Projects. 829.
Loma Linda University Electronic Theses and Dissertations
Loma Linda University. Del E. Webb Memorial Library. University Archives