Abstract

A poisonous steroid-type compound was isolated from Centaurea repens. After extraction with absolute ethanol, removal of the solvent, addition of 2% HCl to precipitate the pigments, and filtering, the pH was adjusted to 9 with aqueous anunonia. This solution was exhaustively extracted with chloroform, evaporated to dryness, and extracted with ethyl ether.

This fraction was chromatographed on silica-gel plates, using 10:1 1,2-dichloroethane-methanol as the developer. The band at Rf 4.2 was removed and found toxic to mice. Labored, slow, gasping respiration and frequent involuntary muscular contractions became apparent within a few minutes following the intraperitoneal injection into each animal. Death resulted after sufficient dosages.

Further separation was achieved by subjecting the Rf 0.42 toxic band to a second development in 55:6 1,2-dichloroethane-methanol. A compound appeared at Rf 0.54 which gave positive color tests for steroid structure with o-phosphoric acid, and 1:50:50 (w/v/v) vanillin-phosphoric acid-water reagents.

LLU Discipline

Biochemistry

Department

Chemistry

School

Graduate School

First Advisor

William D. Leech

Second Advisor

Maurice E. Mathisen

Third Advisor

George J. Nelson

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1966

Date (Title Page)

8-1966

Language

English

Library of Congress/MESH Subject Headings

Poisonous plants; Thistle

Type

Thesis

Page Count

v; 19

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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