Abstract

The time delayed complementation of plant proteins was tested in weanling Sprague-Dawley male rats by measuring growth rates as well as plasma and tissue free amino acids. Diets of pinto beans with rice, corn or wheat were pair-fed in combination or alternating 4 times a day at 6 and 11 am, and 4 and 9 pm for 4 weeks. Plasma, liver and muscle samples were obtained 2 hours postprandial, a time determined most suitable for sacrifice, and analyzed for free amino acids via ion-exchange chromatography. There were no significant differences in growth of rats fed combination or alternating diets.during week 3 and 4 with the exception of the limiting corn-bean combination and alternating diets in week four. Step-wise multiple regression of the data show plasma free amino acids most correlated to growth included asparagine, 15.1% contribution; and arginine, tyrosine, histidine, 1- methylhistidine and cystathionine, each contributing <5%. The following amino acids were negatively correlated with growth: threonine, 29.2%; and leucine, phosphoethanolamine, taurine and serine <6.5%. This study confirms previous work in this laboratory which suggested that vegetable proteins fed 5 hours apart in combined or alternating fashion had equal growth promoting capacity. Contrary to expectations, the limiting amino acids in these diets -- methionine, lysine and tryptophan -- were not the postprandial plasma amino acids most related to growth rate. More study is necessary to correlate the limiting amino acids in proteins and tissue free amino acids with the growth rate of the rat.

Department

Nutrition

School

Graduate School

First Advisor

Albert Sanchez

Second Advisor

Richard Hubbard

Third Advisor

Ulma D. Register

Fourth Advisor

Gerald Shavlik

Degree Name

Master of Science (MS)

Degree Level

M.S.

Year Degree Awarded

1987

Date (Title Page)

12-1987

Language

English

Library of Congress/MESH Subject Headings

Complement; Vegetable Proteins; Time.

Type

Thesis

Page Count

iii; 27

Digital Format

PDF

Digital Publisher

Loma Linda University Libraries

Usage Rights

This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Collection

Loma Linda University Electronic Theses and Dissertations

Collection Website

http://scholarsrepository.llu.edu/etd/

Repository

Loma Linda University. Del E. Webb Memorial Library. University Archives

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