The use of a cation exchange resin in decalcification

Document Type

Article

Publication Date

1-1-1953

Publication Title

Biotechnic and Histochemistry

ISSN

10520295

Abstract

Decalcification of 2-3 mm. sections of human ribs by 5-40% concentrations of both formic and nitric acid was subjected to a controlled study. The effect of adding 1 g. of phloroglucinol and of 2.5, 5 and 10 g. of the exchange resin (Win-3000) per 100 ml. of decalcifying solution was observed after staining celloidin sections with hematoxylin and Triosin or with Giemsa stain. Electrolytically decalcified material was included for comparison also. The following conclusions were drawn: (1) The addition of resin did not appreciably shorten the decalcification time nor enhance tissue preservation and staining qualities. (2) Formic acid, 20% or less, gave results superior to nitric acid in any concentration and also superior to those obtained after the electrolytic method. (3) The addition of 1% phloroglucinol to formic acid solution improved both preservation and staining. (4) Helly's fluid fixation with all combinations gave uniformly better results than formalin fixation. © 1953 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Volume

28

Issue

3

First Page

155

Last Page

158

DOI

10.3109/10520295309105119

PubMed ID

13056822

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